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Since most of the relevant studies focus on animal models, which have significant physiological differences from humans, leading to a scarcity of clinical data and insufficient evidence for the safety of young blood in clinical applications

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The GSH/GSSG content and ATP levels of 0.51 10 5 cells/well were measured by a luminescent-based assay (Promega V6612 for GSH/GSSG and Promega G7571 or Sigma MAK135 for ATP) following the manufacturers protocol